Applications:Suitable for use in Immunoblot, ELISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:5000-1:25,000.
1.0ug of Goat IgG was used in a standard capture ELISA using pNPP (p-nitrophenyl phosphate) as a substrate for 30 minutes at RT
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C as an undiluted liquid for 12 months.
Dilute only prior to immediate use.
DO NOT FREEZE.
Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.05M Tris chloride, 0.15M sodium chloride, 0.001M magnesium chloride, 0.0001M zinc chloride, 50% glycerol, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide. Labeled with Alkaline Phosphatase (MW: 140kD).
Specificity：Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Rabbit Serum, Mouse IgG and Mouse Serum. No reaction was observedagainst anti-Pepsin, anti-Rabbit IgG F(c) or Human Serum Proteins.
