Applications:Suitable for use in Western Blot, ELISA and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 1:2000-1:20,000ELISA: 1:130,000Immunohistochemistry: 1:500-1:2500Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C after receipt.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Labeled with horseradish peroxidase (HRP).
Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
HRP conjugates are sensitive to light.
仕様
Size：500ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% Gentamicin Sulfate. Labeled with Horseradish Peroxidase (HRP). Reconstitute with 500ul sterile ddH2O.
Specificity：Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-goat serum, mouse IgG, mouse IgG F(ab’)2 and mouse serum. No reaction was observed against anti-pepsin, anti-goat IgG F(c), mouse IgG F(c) or bovine, equine, human, rabbit, rat or sheep serum proteins.
