Applications:Suitable for use in Western and Dot Blot, ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:10,000ELISA: 1:10,000-1:50,000Immunohistochemistry: 1:500-1:2500Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Labeled with horseradish peroxidase (HRP).
Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
HRP conjugates are sensitive to light.
仕様
Size：500ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate. Labeled with HRP. Reconstitute with 500ul sterile ddH2O.
Specificity：Recognizes mouse IgG. Assay by IEP resulted in a single precipitin arc against anti-peroxidase, anti-goat serum, mouse IgG and mouse serum. No reaction was observed against anti-pepsin or anti-goat IgG F(c).
