Applications:Suitable for Western Blot, ELISA and Immunohistochemistry.
Other applications not tested
Recommended Dilutions: ELISA: 1:4000-1:100,000.1.0 ug of mouse IgG was used in a standard capture ELISA using S7973-97A (Streptavidin (HRP)) and A0030 (ABTS Stable Liquid Substrate 1.46mM for HRPO) as a substrate for 30 minutes at RT
Western Blot: 1:2000-1:10,000Immunohistochemistry: 1:1000-1:5000Optimal dilution to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：500ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Biotin.
Specificity：Recognizes mouse IgG F(ab’)2 fragment. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-goat serum, mouse IgG, mouse IgG F(ab’)2 and mouse serum. Does not crossreact with anti-pepsin, anti-goat IgG F(c), mouse IgGF(c). Species Crossreactivity: Does not crossreact with bovine, equine, human, rabbit, rat and sheep serum proteins.
