Applications:Suitable for use in Western Blot, ELISA, Immunohistochemistry and Immunomicroscopy Other applications not tested.
Recommended Dilution:ELISA: 1:20,000 to 1:100,000 30 minutes at RT
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:2mg
Host:rabbit
Source Antibody:mouse
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2, 15mg/ml BSA (IgG and Protease free), 0.01% sodium azide, 0.01% thimerosal, 40% glycerol. Labeled with Biotin. Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule.
Specificity:Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Mouse IgG, Mouse IgG F(ab’)2 and Mouse Serum. No reaction was observed against Mouse IgG F(c).