Applications:Suitable for use in Immunoblot (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilution:ELISA: 1:20,000-1:80,000Western Blot: 1:20,000-1:80,000Immunohistochemistry: 1:20,000-1:80,000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Reconstitute to nominal volume by adding sterile 40-50% glycerol.
Aliquot and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：500ug
Host：rabbit
Source Antibody：sheep
Grade：Affinity Purified
Purity：Purified by Immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease Free), 0.01% gentamicin sulfate. Labeled with HRP. Reconstitute in 500ul sterile water or 40-50% glycerol.
Specificity：Recognizes sheep IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Rabbit Serum. No reaction was observed against anti-Papain or anti-Rabbit IgG F(c).
