Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG Fab molecule
Applications:Suitable for use in immunoblotting, ELISA, immunohistochemistry, immunomicroscopy.
Other applications have not been tested.
Recommended Dilution:ELISA: 1:15,000 to 1:60,000 Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:500ug
Host:rabbit
Source Antibody:sheep
Grade:Affinity Purified
Purity:Purified by Immunoaffinity chromatography
Form:Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease Free), 0.01% sodium azide. Labeled with Biotin.
Specificity:Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin and anti-Rabbit Serum. No reaction was observed against anti-Papain or anti-Rabbit IgG F(c).