Applications:Suitable for use in FLISA (Fluorescent), Western Blot and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:500ul
Host:rabbit
Source Antibody:sheep
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a lyophilized powder in PBS, pH 7.2, 0.09% sodium azide, 1% BSA. Labeled with fluorescein isothiocyanate (MW: 390D) (FITC). Absorption Wavelength: 495nm. Emission Wavelength: 528nm. Fluorochrome/Protein Ratio: 2.5moles FITC per mole of rabbit IgG. Reconstitute with 0.5ml sterile, distilled, deionized water or sterile 40-50% glycerol.
Specificity:Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Sheep IgG, Sheep IgG F(c) and Sheep Serum. No reaction was observed against Sheep IgG F(ab’)2 or Human Serum Proteins.