Applications:Suitable for Immunoblot, ELISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:ELISA: 1:3000 to 1:14,000 using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature
Optimal dilution determined by the researcher.
Storage and Stability:Store at 4°C.
Do not freeze.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1mg
Host：donkey
Source Antibody：sheep
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, pH 8.0, 10mg/ml BSA (IgG and Protease free), 0.01% Sodium Azide, 50% Glycerol.
Specificity：Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Donkey Serum, Sheep IgG and Sheep Serum. No reaction was observed against Chicken, guinea pig, Hamster, Horse, Human, Mouse, Rabbit and Rat Serum Proteins.
