Applications:Suitable for use in ELISA, Immunohistochemistry, Western Blot, Dot Blot and Immunoperoxidase Electron Microscopy.
Other applications not tested.
Recommended Dilutions:ELISA: 1:10,000 to 1:100,000 Immunohistochemistry: 1:500-1:5000Western Blot: 1:1000-1:10,000Optimal dilution determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer. Labeled with horseradish peroxidase (HRP).
Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
HRP conjugates are sensitive to light.
仕様
Size：500ul
Host：goat
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.6, 15mg/ml BSA (IgG and Protease free). Preservative free. Labeled with Horseradish Peroxidase (HRP). Reconstitute with 500ul sterile ddH2O.
Specificity：Recognizes rat IgG, F(ab’)2. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rat IgG, Rat IgG F(ab’)2 and Rat Serum. No reaction was observed against anti-pepsin, anti-goat IgG F(c), rat IgG F(c) or bovine, horse and human serum proteins.
