Applications:Suitable for use in FLISA and Immunofluorescence as well as other antibody based fluorescent assays.
Other applications have not been tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Absorption Wavelength: 596nm Emission Wavelength: 620nmFluorochrome/Protein Ratio: 2.5 moles Texas Red™ per mole of Goat IgG F(ab’)2
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:1mg
Host:goat
Source Antibody:rat
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Form:Supplied as a lyophilized powder from PBS, pH 7.2, 15mg/ml BSA (IgG and Protease free), 0.05% Sodium Azide. Label: Texas Red™ Sulfonyl Chloride (MW 625 daltons)
Specificity:Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG F(ab')2 and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), anti-Rat F(c) or Bovine, Horse, and Human Serum Proteins.