Applications:Suitable for Immunoblot, ELISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:ELISA: 1:1000 to 1:5000 using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature
Optimal dilution determined by the researcher.
Storage and Stability:Store at 4°C.
Do not freeze.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：500ug
Host：goat
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, pH 8.0, 10mg/ml BSA (IgG and Protease free), 0.01% Sodium Azide, 50% Glycerol.
Specificity：Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Rat IgG and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins.
