Applications:Suitable for use in ELISA, Immunoblot (Western or Dot Blot), Immunohistochemistry and Immunomicroscopy.
Other applications not tested.
Recommended Dilutions:ELISA: 1:10,000-1:40,000Western Blot: 1:1000-1:4000 Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1mg
Host：goat
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography followed by solid phase adsorption to remove unwanted reactivities, papain digestion and chromatographic separation.
Form：Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% Gentamicin Sulfate. Label: Horseradish Peroxidase (HRP) (MW: 40kD). Reconstitution: Reconstitute with 1ml sterile dH2O or sterile 40-50% glycerol for long term storage.
Specificity：Recognizes rat IgG, H&L. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase and anti-goat serum. No reaction was observed against anti-papain or anti-goat IgG F(c).
