Applications:Suitable for use in FLISA and Immunofluorescence as well as other antibody based fluorescent assays.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Absorption Wavelength: 495nmEmission Wavelength: 528nmFluorochrome/Protein Ratio: 2.8moles FITC per mole of goat IgG, F(ab’)2.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1mg
Host：goat
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, pH 7.2, 0.09% sodium azide, 1% BSA. Label: Fluorescein isothiocyanate (MW: 390D) (FITC). Reconstitute with 1ml sterile, distilled, deionized water or sterile 40-50% glycerol.
Specificity：Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Rat IgG, Rat IgG F(c) and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(ab’)2 or Bovine, Horse and Human Serum Proteins
