Applications:Suitable for use in FLISA, Flow Cytometry and Immunofluorescence.
Other applications not tested.
Recommended Dilution:FLISA:1:10,000 - 1:50,000Flow Cytometry: 1:500 - 1:2,500IF Microscopy: 1:1,000 -1:5,000Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C before and after reconstitution.
DO NOT FREEZE! Lyophilized and reconstituted products are stable for 12 months at 4°C after receipt.
Reconstitute lyophilized powder with sterile ddH2O prior to use and store remainder as an undiluted liquid.
Further dilutions can be made in assay buffer.
Caution: APC conjugates are sensitive to light.
For maximum recovery of product centrifuge the original vial prior to removing the cap.
仕様
Size:1mg
Host:goat
Source Antibody:rabbit
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Label: Texas Red™ Sulfonyl Chloride (MW: 625D). Absorption Wavelength: 596nm Emission Wavelength: 620nm. Fluorochrome/Protein Ratio: 1.0mole Texas Red™ per mole of goat IgG Fab. Reconstitute with 1ml sterile deionized water.
Specificity:Recognizes rabbit IgG (H&L). Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum. No reaction was observed against anti-pepsin or anti-goat IgG F(c).