Applications:Suitable for Immunoblot, ELISA, Immunohistochemistry and Immunomicroscopy.
Other applications not tested
Recommended Dilutions: ELISA: 1:9000 to 1:36,000, 30 minutes at RT
Optimal dilution determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:500ug
Host:goat
Source Antibody:rabbit
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA, 0.01% sodium azide. Reconstitute with 0.5ml sterile, distilled, deionized water or sterile 40-50% glycerol. Labeled with Biotin. Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule
Specificity:Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse, Human, Mouse, Rat and Sheep Serum Proteins.