Applications:Suitable for use in FLISA (Fluorescent) (Fluorescent) and Immunofluorescence as well as other antibody based fluorescent assays.
Other applications not tested.
Recommended Dilutions:FLISA (Fluorescent): 1:10,000-1:50,000Immunofluorescence Microscopy: 1:1000-1:5000Optimal dilutions to be determined by the researcher.
Fluorochrome/Protein Ratio:1.6moles FITC per mole of Donkey IgG F(ab)
Absorption Wavelength: 495nm
Emission Wavelength: 528nm
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1mg
Host：donkey
Source Antibody：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Fluorescein Isothiocyanate (FITC). Reconstitute with 1ml sterile ddH2O.
Specificity：Recognizes rabbit IgG. Assay by IEP resulted in a single precipitin arc against anti-Fluorescein and anti-donkey serum. No reaction was observed against anti-pepsin or anti-donkey IgG F(c).
