Applications:Suitable for use in FLISA, Flow Cytometry and Immunofluorescence.
Other applications not tested.
Recommended Dilution:FLISA:1:10,000 - 1:50,000Flow Cytometry: 1:500 - 1:2,500IF Microscopy: 1:1,000 -1:5,000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder is stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O prior to use.
To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1mg
Host：goat
Source Antibody：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Label: Fluorescein isothiocyanate (FITC) (MW: 390D). Reconstitute with 1ml sterile dH2O. Absorption Wavelength: 495nm Emission Wavelength: 528nm. Fluorochrome/Protein Ratio: 3.4 moles FITC per mole of Goat IgG Fab.
Specificity：Recognizes rabbit IgG (H&L). Assay by immunoelectrophoresis resulted in a single precipitin arcagainst anti-Fluorescein and anti-Goat Serum. No reaction was observed against anti-Pepsin or anti-Goat IgGF(c).
