Applications:Suitable for use in ELISA, Western Blot, Dot Blot, Immunoelectrophoresis, Immunohistochemistry and Immunoperoxidase Electron Microscopy.
Other applications not tested.
Recommended Dilution:ELISA: 1:10.000-1:50,000Western Blot: 1:1,000-1:5,000Immunohistochemistry: 1:500-1:2,500Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:1mg
Host:goat
Source Antibody:rabbit
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography
Form:Supplied as a powder lyophilized from a solution containing PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), and 0.01% gentamicin sulfate. Labeled with HRP. Reconstitute with 1ml sterile ddH2O.
Specificity:Recognizes rabbit IgG, Fab. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase and anti-goat serum. No reaction was observed against anti-papain or anti-goat IgG F(c).