Applications:Suitable for use in FLISA and Immunofluorescence as well as other antibody based fluorescent assays.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Absorption Wavelength:495nm
Emission Wavelength:528nm
Fluorochrome/Protein Ratio:3.5 moles DTAF per mole of Goat IgG
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:1mg
Host:goat
Source Antibody:rabbit
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.4, 10mg/ml BSA (IgG and Protease free), 0.01% Thimerisol. Labeled with Fluorescein isothiocyanate (FITC).
Specificity:Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-goat serum, rabbit IgG, rabbit IgG F(c) and rabbit serum. No reaction was observed against rabbit IgG F(ab’)2.