Applications: Suitable for use in Immunoblot (Western or Dot Blot), ELISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions: ELISA: 1:6300Western Blot: 1:200-1:1000Immunohistochemistry: 1:100-1:500Optimal dilution determined by the researcher.
Storage and Stability:May be stored at 4°C as an undiluted liquid for 12 months.
Dilute only prior to immediate use.
DO NOT FREEZE.
Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:500ug
Host:goat
Source Antibody:mouse
Grade:Affinity Purified
Purity:Purified by Immunoaffinity chromatography.
Form:Supplied as a liquid in 0.05M Tris Chloride, 0.15M sodium chloride, 0.001M magnesium chloride, 0.0001M zinc chloride, pH 8.0, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide, 50% glycerol. Labeled with Alkaline Phosphatase.
Specificity:Recognizes mouse IgG, IgA, IgM. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase and anti-goat serum. No reaction was observed against anti-pepsin, anti-goat IgG F(c) or human serum proteins.