Applications:Suitable for Western Blot, ELISA, Flow Cytometry, Immunohistochemistry and Immunomicroscopy.
Other applications not tested
Recommended Dilutions: ELISA: 1:1,200,000Western Blot: 1:5000-1:20,000Immunohistochemistry: 1:2000-1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Form：Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Reconstitute with 1ml sterile dH2O. Labeled with Biotin.
Specificity：Recognizes mouse IgG, IgA and IgM, whole molecules (all subclasses, isotypes and chain combinations). Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-goat serum. Species Crossreactivity: Does not crossreact with human serum protein.
