Applications:Suitable for use in Immunoblot, ELISA, immunomicroscopy and immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:50,000-1:200,000; 1.0ug of Goat IgG was used in a standard capture ELISA with S7973-97A and ABTS (A0030) as a substrate for 30 minutes at RT
Optimal dilutions to be determined by the researcher.
Biotin/Protein Ratio:10-20 BAC molecules per Chicken IgG molecule
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol, dH2O.
Aliquot and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1mg
Host：chicken
Source Antibody：goat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Reconstitute with 1ml sterile 40-50% glycerol, dH2O.
Specificity：Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Chicken Serum, Goat IgG and Goat Serum.
