Applications:Suitable for use in Immunoblot, ELISA, immunomicroscopy and immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:8000-1:36,000; 1ug of Goat IgG was used in a standard capture ELISA with Streptavidin (HRP) (S7973-97A) and ABTS as a substrate for 30 minutes at RT
Optimal dilutions to be determined by the researcher.
Biotin/Protein Ratio:10-20 BAC molecules per Donkey IgG Fab molecule
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1mg
Host：donkey
Source Antibody：goat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with biotin. Reconstitute with 1ml sterile 40-50% glycerol, dH2O.
Specificity：Recognizes goat IgG Fab. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-donkey serum. No reaction was observed against anti-papain or anti-donkey IgG F(c).
