Applications:Suitable for use in Western Blot, Dot Blot, ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilution:ELISA: 1:8000-1:32,000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:1mg
Host:mouse
Source Antibody:rabbit
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% Gentamicin Sulfate. Labeled with HRP (MW: 40kD). Reconstitute with 1ml sterile, distilled, deionized water or sterile 40-50% glycerol for long term storage.
Specificity:Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-mouse serum, rabbit IgG and rabbit serum. No reaction was observed against goat, human and mouse serum proteins.