Applications:Suitable for Immunoblot, ELISA, Immunohistochemistry and Immunomicroscopy.
Other applications not tested
Recommended Dilution: ELISA: 1:25,000 to 1:100,000, using peroxidase conjugated streptavidin and ABTS for 30 minutes at RT
Optimal dilution determined by the researcher.
Biotin/Protein Ratio:10-20 BAC molecules per donkey IgG molecule.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：500ug
Host：donkey
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA, 0.01% sodium azide. Reconstitute with 0.5ml sterile, distilled, deionized water or sterile 40-50% glycerol. Labeled with Biotin.
Specificity：Assay by IEP resulted in a single precipitin arc against anti-biotin, anti-donkey serum, mouse IgG and mouse serum. No reaction to anti pepsin, anti donkey IgG F(c) or bovine, chicken, goat, guinea pig, hamster, equine, human, rabbit, rat and sheep serum proteins.
