Applications:Suitable for Western Blot, ELISA, Immunohistochemistry and Immunomicroscopy.
Other applications not tested
Recommended Dilutions: Western Blot: 1:2000-1:10,000ELISA: 1:17,000-1:1:100,000.
Assayed against 1ug of mouse IgG in a standard capture ELISA using ABTS as a substrate for 30 minutes at RT
Immunohistochemistry: 1:1000-1:5000Optimal dilution determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：500ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Reconstitute with 500ul sterile ddH2O. Labeled with Biotin.
Specificity：Recognize mouse IgG, H&L. Assay by IEP resulted in a single precipitin arc against anti-biotin, anti-rabbit serum, mouse IgG and mouse serum. No reaction was observed against anti-pepsin, anti-rabbit IgG F(c) or human serum proteins
