Applications:Suitable for use in Western Blot, ELISA and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 1:5000-1:100,000ELISA: 1:5000-1:100,000Immunohistochemistry: 1:500-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder is stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O prior to use.
To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ul
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from 0.01M sodium phosphate, 0.25M sodium chloride, pH 7.6, 15mg/ml BSA (IgG and Protease free). Labeled with Horseradish peroxidase (HRP). Reconstitute with 500ul sterie ddH2O.
Specificity：Recognizes human IgA, alpha. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-goat serum. No reaction was observed against anti-pepsin or anti-goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other human heavy or light chain isotypes.
