Applications:Suitable for use in Western Blot, ELISA, Immunohistochemistry and Immunomicroscopy.
Other applications have not been tested.
Recommended Dilution:ELISA: 1:20,000 to 1:400,000 Western Blot: 1:20,000-1:400,00Immunohistochemistry (tissue sections): 1:500-1:5000 Optimal dilutions to be determined by the researcher.
Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：500ul
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography using Human IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Form：Supplied as a lyophilized powder from PBS, pH 7.6, 15mg/ml BSA (IgG and Protease free), 0.01% Sodium Azide, 0.01% Thimerosal.
Specificity：Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Human IgA and Human Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or other human heavy or light chain proteins.
