Applications:Suitable for use in FLISA, Western Blot, Flow Cytometry, Immunomicroscopy and Immunofluorescence.
Other applications have not been tested.
Recommended Dilutions:FLISA: 1:10,000-1:50,000Immunofluorescence: 1;100-1:5000Optimal dilutions to be determined by the researcher.
Excitation Wavelength: 495nm
Emission Wavelength: 528nm
Fluorochrome/Protein Ratio: 1.3 moles FITC per mole of Rabbit IgG Fab
Storage and Stability:Lyophilized powder is stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O prior to use.
To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Reconstitute with 500ul sterile dH2O.
Specificity：Recognizes human IgG (H+L). Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein and anti-rabbit serum. No reaction was observed against anti-papain or anti-rabbit IgG F(c).
