Applications:Suitable for use in Western Blot, ELISA, Immunohistochemistry and Immunomicroscopy.
Other applications have not been tested.
Recommended Dilution:ELISA: 1:50,000-1:1,000,000 Western Blot: 1:10,000-1:100,000 Immunohistochemistry (tissue sections)1:500-1:5000Optimal dilutions to be determined by the researcher.
Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：500ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography using Human IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Form：Supplied as a lyophilized powder from PBS, pH 7.2, 15 mg/ml Bovine Serum Albumin (BSA) IgG and Protease freePreservative: 0.05% Sodium Azide, 0.01% Thimerosal
Specificity：Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Human IgM Fc5µ and Human Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or other human heavy or light chain proteins.
