Applications:Suitable for use in Western Blot, ELISA and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:ELISA: 1:12,000- 175,000.
Assayed against 1ug of human IgG in a standard capture ELISA using S7973-97A and ABTS as a substrate for 30 minutes at RT
Western Blot: 1:2000-1:10,000Immunohistochemistry: 1:1000-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1mg
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Biotin. Reconstitute with 1ml sterile ddH2O.
Specificity：Recoghnizes human IgG, Fc. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-goat serum, human IgG, human IgG F(c) and human serum. No reaction was observed against anti-pepsin, anti-goat IgG F(c), human IgG F(ab’)2 or bovine, equine, mouse or rat serum proteins.
