Applications:Suitable for use inFLISA and Immunofluorescence.
Other applications have not been tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Label:FITC MW: 390D
Absorption Wavelength: 495 nm
Emission Wavelength: 528 nm
Fluorochrome/Protein Ratio: 2.8 moles FITC per mole of Goat IgG F(ab’)2
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1mg
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography using Human IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Form：Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free),0.05% Sodium Azide, 0.01% Thimerosal
Specificity：Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Human IgM and Human Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity againstother human heavy or light chain isotypes
