Applications:Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electronmicroscopy and immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:200-1:1000 30 minutes at RT
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1mg
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a lyophilized powder from 0.125M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA, pH 8.0, 10 mg/ml BSA (IgG and Protease free), 0.01% Gentamicin Sulfate. Labeled with horseradish peroxidase (HRP) (MW:40kD).
Specificity：Assay by IEP resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. Noreaction was observed against anti-Pepsin or anti-Goat IgG F(c). <1% cross reactivity against other human heavy or light chain isotypes.
