Applications:Suitable for use in Western Blot, ELISA, Immunohistochemistry and Immunomicroscopy.
Other applications have not been tested.
Recommended Dilution:ELISA: 1:30,000-1:130,000; Antibody was assayed against 1ug of human IgG in a standard capture ELISA using Streptavidin (HRP) (Cat.
#S7973-97A) and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at RT
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1mg
Host：donkey
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide, 40% glycerol. Labeled with Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC). Biotin/Protein Ratio: 10-20 BAC molecules per Donkey IgG molecule.
Specificity：Recognizes human IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-donkey serum, human IgG and human serum.
