Applications:Suitable for use in Western Blot, ELISA, Immunohistochemistry and Immunomicroscopy.
Other applications have not been tested.
Recommended Dilution:Western Blot: 1:2000-1:10,000ELISA: 1:200,000Immunohistochemistry: 1:1000-1:5000Optimal dilutions to be determined by the researcher.
Biotin/Protein Ratio:10-20 BAC molecules per goat IgG molecule
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1mg
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Reconstitute with 1ml sterile dH2O. Labeled with Biotin.
Specificity：Recognizes the kappa chain subunit of IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-goat serum. Specificity was confirmed by ELISA at less than 1% crossreactivity against other human heavy or light chain proteins.
