Applications:Suitable for use in Western Blot, ELISA and immunohistochemistry.
Other applications not tested.
Recommended Dilutions:ELISA: 1:2000-1:10,000Western Blot: 1:500-1:2500Immunohistochemistry: 1:200-1:1000Optimal dilution determined by the researcher.
Storage and Stability:May be stored at 4°C before opening.
DO NOT FREEZE! Stable at 4°C as an undiluted liquid.
Dilute only prior to immediate use.
Stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Freezing alkaline phosphatase conjugates will result in a substantial loss of activity.
仕様
Size：1mg
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.05M Tris chloride, 0.15M sodium chloride, pH 8.0, 0.001M magnesium chloride, 0.0001M zinc chloride, 10mg/ml BSA (IgG and protease free), 0.09% sodium azide, 50% glycerol. Labeled with Alkaline Phosphatase (AP).
Specificity：Recognizes human IgG H&L. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine), anti-goat serum, human IgG and human serum. No reaction was observed against bovine, chicken, goat, guinea pig, hamster, equine, mouse, rabbit, rat and sheep serum proteins.
