Applications:Suitable for use in ELISA, Immunohistochemistry and Western Blot.
Other applications have not been tested.
Recommended Dilution:ELISA: 1:3000-1:12,000Immunohistochemistry: 1;200 -1:1,000Western Blot: 1:500 -1;2,000Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
仕様
Size：500ug
Host：rabbit
Source Antibody：hamster
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography using Hamster IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Form：Supplied as a sterile-filtered liquid (pH 8) in 0.05 M Tris-chloride, 0.15M sodium chloride, 0.001M magnesium chloride, 0.0001M zinc chloride, 50% (v/v) glycerol, 0.01% sodium azide and 10mg/ml BSA. Labeled with Alkaline Phosphatase (AP).
Specificity：Recognizes Golden Syrian hamster IgG (H&L). Limited cross-reactivity with Armenian hamster IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-rabbit serum, hamster IgG and hamster serum. No reaction was observed against anti-Pepsin or anti-rabbit IgG F(c).
