Applications:Suitable for use in Immunoblot, ELISA, Immunohistochemistry and Immunomicroscopy.
Other applications not tested.
Recommended Dilution:ELISA: 1:25,000-1:150,000.
1.0ug of Golden Syrian Hamster IgG was used in a standard capture ELISA using Streptavidin (HRP) and ABTS (2,2’-azino-bis-[3ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at RT
Optimal dilutions to be determined by the researcher.
Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG Fab molecule
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：500ug
Host：rabbit
Source Antibody：hamster
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide. Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Specificity：Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin and anti-Rabbit Serum. No reaction was observed against anti-Papain or anti-Rabbit IgG F(c).
