Applications:Suitable for use in Immunoblot (Western or Dot blot), ELISA, Immunohistochemistry and Immunoperoxidase electron microscopy.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000-1:40,000.
1.0ug of Golden Syrian Hamster IgG was used in a standard capture ELISA using Streptavidin (HRP) and ABTS (2,2’-azino-bis-[3ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at RT
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1mg
Host：goat
Source Antibody：hamster
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% (w/v) gentamicin sulfate. Label: Horseradish Peroxidase (HRP) (MW 40kD)
Specificity：Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. No reaction was observed against anti-Papain or anti-Goat IgG F(c).
