Applications:Suitable for use in FLISA and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Immunofluorescence Microscopy: 1:500-1:2500Optimal dilutions to be determined by the researcher.
Absorption Wavelength: 495nm Emission Wavelength: 528nm Fluorochrome/Protein Ratio: 2.1 moles FITC per mole of Rabbit IgG F(ab’)2
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:500ul
Host:rabbit
Source Antibody:hamster
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide. Label: Fluorescein isothiocyanate (FITC) (MW 390 daltons)
Specificity:Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Golden Syrian Hamster IgG and Golden Syrian Hamster Serum. No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c). Limited reactivity will occur against Armenian Hamster IgG.