Applications:Suitable for use in Western Blot, ELISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:20,00-1:40,000Immunohistochemistry: 1:200-1:1,000Western Blot: 1:500-1:2,500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C as an undiluted liquid for 12 months.
Dilute only prior to immediate use.
DO NOT FREEZE.
Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:1mg
Host:goat
Source Antibody:hamster
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, pH 8.0, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide, 50% glycerol. Label: Alkaline Phosphatase (Calf Intestine) (MW 140kD )
Specificity:Recognizes Armenian hamster IgG (H&L). Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-goat serum and Armenian hamster serum. Diminished reactivity will occur with Golden Syrian Hamster IgG.