Applications:Suitable for use in Western Blot, ELISA, Immunomicroscopy and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000-1:20,000; Streptavidin (HRP) (S7973-97A) and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6sulfonic acid]) as a substrate for 30 minutes at RT
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1mg
Host：goat
Source Antibody：guinea pig
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% thimerisol, 40% glycerol. Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule. Reconstitute with 1ml sterile, distilled, deionized water or sterile 40-50% glycerol for long term storage.
Specificity：Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, guinea pig IgG and guinea pig Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).
