Applications:Suitable for use in Immunoblot, ELISA, Immunomicroscopy and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:28,000-1:114,000 Streptavidin (HRP) (S7973-97A) and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6sulfonic acid]) as a substrate for 30 minutes at RT
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:2mg
Host:goat
Source Antibody:guinea pig
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide, 40% glycerol. Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule
Specificity:Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, guinea pig IgG, guinea pig IgG F(ab’)2 and guinea pig Serum. No reaction was observed against guinea pig IgG F(c).