Applications:Suitable for use in Western Blot, ELISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:ELISA: 1:1000-1:10,000.
30 minutes at RT
Immunohistochemistry: 1:200-1:1000Western Blot: 1:500-1:2500Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: PE conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
仕様
Size:1mg
Host:goat
Source Antibody:guinea pig
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in 0.05M Tris chloride, 0.15M sodium chloride, 0.001M magnesium chloride, 0.0001M zinc chloride, pH 8.0, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide, 50% glycerol. Labeled with Alkaline Phophatase (AP)
Specificity:Recognizes guinea pig IgG, F(c). Assay by IEP resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, guinea pig IgG, guinea pig IgG F(c) and guinea pig Serum. No reaction was observed against guinea pig IgG F(ab’)2.