Applications:Suitable for use in FLISA, Flow Cytometry and Immunofluorescence.
Other applications not tested.
Recommended Dilutions:FLISA: 1:10,000-1:50,000Immunofluorescence: 1:1,000-1:5000Flow Cytometry: 1:500-1:2500Optimal dilutions to be determined by the researcher.
Absorption Wavelength:495nm Emission Wavelength:528nm Fluorochrome/Protein Ratio: 2.8 moles FITC per mole of Goat IgG
Storage and Stability:Lyophilized powder is stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O prior to use.
To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C.
Reconstituted product is stable for 12 months at -20°C
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
仕様
Size：2mg
Host：goat
Source Antibody：guinea pig
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 10mg/ml BSA, 0.01% sodium azide. Reconstitute in 1ml sterile ddH2O. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, guinea pig IgG, guinea pig IgG F(ab’)2 and guinea pig Serum. No reaction was observed against guinea pig IgG F(c).
