Applications: Suitable for use in Immunoblot (Western or Dot Blot), ELISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions: ELISA: 1:2000-1:10,000
Western Blot: 1:500- 1:2500
Immunohistochemistry: 1:200- 1:1000.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C.
For long-term storage, aliquot and store at 4°C.
Do not freeze.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Further dilutions can be made in assay buffer
仕様
Size：500ug
Host：goat
Source Antibody：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography followed by solid phase adsorption, pepsin digestion and chromatographic separation.
Form：Supplied as a liquid in TBS, 0.001M magnesium chloride, 0.0001M zinc chloride, pH 8.0, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide and 50% glycerol. Label with Alkaline Phosphatase (Calf Intestine) (MW 140kD).
Specificity：Recognizes rabbit IgG, H&L. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-goat serum, rabbit IgG and rabbit serum. No reaction was observed against anti-pepsin, anti-goat IgG F(c) or bovine, equine, human, mouse, rat and sheep serum proteins.
