Applications: Suitable for use in Immunoblot (Western or Dot Blot), ELISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions: ELISA: 1:4500Western Blot: 1:500-1:2500Immunohistochemistry: 1:200-1:1000Optimal dilution determined by the researcher.
Storage and Stability:May be stored at 4°C before opening.
DO NOT FREEZE! Stable at 4°C as an undiluted liquid.
Dilute only pror to immediate use.
Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
AP conjugates are sensitive to light.
仕様
Size:1mg
Host:mouse
Source Antibody:rabbit
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in 0.05M Tris chloride, 0.15M sodium chloride, 0.001M magnesium chloride, 0.0001M zinc chloride, pH 8.0, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide, 50% glycerol. Labeled with Alkaline Phosphatase (AP).
Specificity:Recognizes rabbit IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-mouse serum, rabbit IgG and rabbit Serum. No reaction was observed against goat, human and mouse serum proteins.