Applications:Suitable for Immunoblot, ELISA, Immunohistochemistry and Immunomicroscopy.
Other applications not tested
Recommended Dilution: ELISA: 1:20,000 to 1:80,000, using peroxidase conjugated streptavidin and ABTS for 30 minutes at RT
Optimal dilution determined by the researcher.
Biotin/Protein Ratio:10-20 BAC molecules per sheep IgG molecule.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1mg
Host：sheep
Source Antibody：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA, 0.01% sodium azide. Reconstitute with 1ml sterile, distilled, deionized water or sterile 40-50% glycerol. Labeled with Biotin.
Specificity：Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-sheep serum, rabbit IgG and rabbit serum. No reaction against bovine, chcken, goat, guinea pig, equine, human, mouse, rat and sheep serum proteins.
