Applications:Suitable for Immunoblot, ELISA, Immunohistochemistry and Immunomicroscopy.
Other applications not tested
Recommended Dilutions: ELISA: 1:12,000 to 1:52,000.
Assayed against 1ug raggit IgG in a standard ELISA with ABTS (A0030) as a substrate for 30 minutes at RT
Optimal dilution determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Reconstitute to nominal volume by adding sterile 40-50% glycerol, ddH2O.
Aliquot and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1mg
Host：rat
Source Antibody：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Reconstitute with 1ml sterile 40-50% glycerol, ddH2O. Labeled with Biotin. Biotin/Protein Ratio: 10-20 BAC molecules per rat IgG molecule.
Specificity：Recognizes rabbit IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-rat Serum, rabbit IgG and rabbit serum. No reaction was observed against human, goat and mouse serum proteins.
